Publikationen (peer reviewed)


Integration of Segmented Microflow Chemistry and Online HPLC/MS Analysis on a Microfluidic Chip System Enabling Enantioselective Analyses at the Nanoliter Scale
S. K. Piendl, T. Schönfelder, M. Polack, L. Weigelt, T. van der Zwaag, T. Teutenberg, E. Beckert, D. Belder
Lab Chip 2021, DOI: 10.1039/D1LC00078K

On-chip Mass Spectrometric Analysis in Non-polar Solvents by Liquid Beam Infrared Matrix-Assisted Laser Dispersion/Ionization
R. D. Urban, T. G. Fischer, A. Charvat, K. Wink, B. Krafft, S. Ohla, K. Zeitler, B. Abel, D. Belder
Anal. Bioanal. Chem. 2021, in press, doi: 10.1007/s00216-020-03115-4

Microfluidic device for concentration and SERS‐based detection of bacteria in drinking water
B. Krafft, A. Tycova, R. D. Urban, C. Dusny, D. Belder
Electrophoresis 2021, 42, 86-94.


On-Line Coupling of Chip-Electrochromatography and Ion Mobility Spectrometry
N. T. Hartner, C. R. Raddatz, C. Thoben, S. K. Piendl, S. Zimmermann, D. Belder
Anal. Chem. 2020, 92, 15129-15136.

How electrospray potentials can disrupt droplet microfluidics and how to prevent this
A. J. Peretzki, S. Schmidt, E. Flachowsky, A. Das, R. F. Gerhardt, D. Belder
Lab Chip, 2020, 20, 4456-4465.

In situ monitoring of photocatalyzed isomerization reactions on a microchip flow reactor by IR-MALDI ion mobility spectrometry
C. Prüfert, R. D. Urban, T. G. Fischer, J. Villatoro., D. Riebe., T. Beitz., D. Belder, K. Zeitler, H. G. Löhmannsröben
Anal. Bioanal. Chem. 2020, 412, 7899–7911.

Conversion Efficiencies of a Few Living Microbial Cells Detected at a High Throughput by Droplet-Based ESI-MS
M. Schirmer, K. Wink, S. Ohla, D. Belder, A. Schmid, C. Dusny
Anal. Chem. 2020, 92, 15, 10700–10708.

A visible‐light‐powered polymerization method for the immobilization of enantioselective organocatalysts into microreactors
R. Warias, D. Ragno, A. Massi, D. Belder
Chem. Eur. J. 2020, 26, 13152-13156.

Multiple Heart-Cutting Two-Dimensional Chip-HPLC Combined with Deep-UV Fluorescence and Mass Spectrometric Detection
S. K. Piendl, D. Geissler, L. Weigelt, D. Belder
Anal. Chem.
2020, 92, 3795-3803.

Unravelling the Configuration of Transient ortho-Quinone Methides by Combining Microfluidics with Gas Phase Vibrational Spectroscopy
M. Mayer , M. Pahl , M. Spanka , M. Grellmann , M. Sickert , C. Schneider , K. R. Asmis, D. Belder
Phys. Chem. Chem. Phys. 2020, 22, 4610-4616.

A microfluidic device enabling surface-enhanced Raman spectroscopy at chip-integrated multifunctional nanoporous membranes
B. Krafft, R. P. Selvam, D. Geissler, D. Belder
Anal. Bioanal. Chem. 2020, 412, 267-277.

On-chip integration of normal phase high-performance liquid chromatography and droplet microfluidics introducing ethylene glycol as polar continuous phase for the compartmentalization of n-heptane eluents
A. J. Peretzki, D. Belder
J. Chromatogr. A 2020, 1612, 460653.


Raman Spectroscopic Detection in Continuous Micro-Flow Using a Chip-Integrated Silver Electrode as Electrically Regenerable SERS Substrate
E. M. Höhn, R. Panneerselvam, A. Das, D. Belder
Anal. Chem. 2019, 91, 9844-9854.

2D in Seconds: Coupling of Chip-HPLC with Ion Mobility Spectrometry
S. K. Piendl, C. R. Raddatz, N. T. Hartner, C. Thoben, R. Warias, S. Zimmermann, D. Belder
Anal. Chem. 2019, 91, 7613-7620.

Non-aqueous micro free-flow electrophoresis for continuous separation of reaction mixtures in organic media
B. M. Rudisch, S. A. Pfeiffer, D. Geissler, E. Speckmeier, A. A. Robitzki, K. Zeitler, D. Belder
Anal. Chem. 2019, 91, 6689-6694.

Supercritical fluid chromatography on-chip with two-photon excited fluorescence detection for high-speed chiral separations
J. J. Heiland, D. Geissler, S. K. Piendl, R. Warias, D. Belder
Anal. Chem. 2019, 91, 6134-6140.

Large‐Ring Cyclodextrins as Chiral Selectors for Enantiomeric Pharmaceuticals
C. Sonnendecker, S. Thürmann, C. Przybylski, F. D. Zitzmann, N. Heinke, Y. Krauke, K. Monks, A. A. Robitzki, D. Belder, W. Zimmermann
Angew. Chem. Int. Ed. 2019, 58, 6411-6414.

Joining Microfluidics with Infrared Photodissociation: Online Monitoring of Isomeric Flow-Reaction Intermediates
M. Pahl, M. Mayer, M. Schneider, D. Belder, K. R. Asmis
Anal Chem. 2019, 91, 3199-3203.

Fluorescence lifetime-activated droplet sorting in microfluidic chip systems
S. Hasan, D. Geissler, K. Wink, A. Hagen, J. J. Heiland, D. Belder
Lab Chip 2019, 19, 403-409.


An integrated lab‐on‐a‐chip approach to study heterogeneous enantioselective catalysts at the microscale
R. Warias, A. Zaghi, J. J. Heiland, S. K. Piendl, K. Gilmore, P. H. Seeberger, A. Massi, D. Belder
2018, 10, 5382 - 5385.

An integrated chip-mass spectrometry and epifluorescence approach for online monitoring of bioactive metabolites from incubated Actinobacteria in picoliter droplets
K. Wink, L. Mahler, J. R. Beulig, S. K. Piendl, M. Roth, D. Belder
Anal. Bioanal. Chem. 2018, 410, 7679-7687.

Sheathless coupling of microchip electrophoresis to ESI-MS utilising an integrated photo polymerised membrane for electric contacting
T. Scholl, C. Dietze, M. Schmidt, S. Ohla, D. Belder
Anal. Bioanal. Chem. 2018, 410, 5741-5750.

Detection of antibiotics synthetized in microfluidic picolitre-droplets by various actinobacteria
L. Mahler, K. Wink, R. J. Beulig, K. Scherlach, M. Tovar, E. Zang, K. Martin, C. Hertweck, D. Belder, M. Roth
Scientific reports 2018, 8, 13087.

Analyte and matrix evaporability–key players of low-temperature plasma ionization for ambient mass spectrometry
A. Kiontke, C. Engel, D. Belder, C. Birkemeyer
Anal. Bioanal. Chem. 2018, 410, 5123-5130.  

The requirements for low-temperature plasma ionization support miniaturization of the ion source
A. Kiontke, F. Holzer, D. Belder, C. Birkemeyer
Anal. Bioanal. Chem. 2018, 410, 3715-3722.

Surface enhanced Raman spectroscopy in microchip electrophoresis
A. Tycova, R. F. Gerhardt, D. Belder
J. Chromatogr. A 2018, 1541, 39-46.

Catalysis by Metal Nanoparticles in a Plug-in Optofluidic Platform: Redox Reactions of p-Nitrobenzenethiol and p-Aminothiophenol
Z. Zhang, U. Gernert, R. Gerhardt, E.-M. Höhn, D. Belder J. Kneipp
ACS Catal.
2018, 8, 2443–2449.

Continuous purification of reaction products by micro free-flow electrophoresis enabled by large area deep-UV fluorescence imaging
S. A. Pfeiffer, B. M. Rudisch, P. Glaeser, M. Spanka, F. Nitschke, A. A. Robitzki, C. Schneider, S. Nagl, D. Belder
Anal. Bioanal. Chem.
2018, 410, 853-862.

A chip-integrated optical microfluidic pressure sensor
C. Hoera, A. Kiontke, M. Pahl, D. Belder
Sensors and Actuators B: Chemical
2018, 255, 2407-2415.


Microfluidic Free-Flow Electrophoresis Based Solvent Exchanger for Continuously Operating Lab-on-Chip Applications
F. D. Zitzmann, H. G. Jahnke, S. A. Pfeiffer, R. Frank, F. Nitschke, L. Mauritz, B. Abel, D. Belder, A. A. Robitzki, Anal. Chem. 2017, 89, 13550–13558

Seamless combination of high pressure chip-HPLC and droplet microfluidics on an integrated microfluidic glass chip
R. Gerhardt, A. J. Peretzki, S. K. Piendl, D. Belder, Anal. Chem. 2017, 89, 13030-13037

A novel microfluidic microelectrode chip for a significantly enhanced monitoring of NPY-receptor activation in live mode
F. Zitzmann, H. G. Jahnke, F. Nitschke, A. G. Beck-Sickinger, B. Abel, D. Belder, A. Robitzki, Lab Chip 2017, 17, 4294

Liquid beam desorption mass spectrometry for the investigation of continuous flow reactions in microfluidic chips
S. Schulze, M. Pahl, F. Stolz, J. Appun, B. Abel, C. Schneider, D. Belder, Anal. Chem. 2017, 89, 6175–6181

A Highly Stereoselective Synthesis of Tetrahydrofurans
J. Appun, M. Boomhoff, P. Hoffmeyer, I. Kallweit, M. Pahl, D. Belder, C. Schneider, Angew. Chem. Int. Ed. 2017, 24, 6862-6865

A droplet-chip / mass spectrometry approach to study organic synthesis at nanoliter scale
J. R. Beulig, R. Warias, J. J. Heiland, S. Ohla, K. Zeitler, D. Belder, Lab Chip 2017, 17, 1996-2002

Temperature gradient elution and superheated eluents in chip-HPLC
J. J. Heiland, C. Lotter, V. Stein, L. Mauritz, D. Belder, Anal. Chem. 2017, 89, 3266–3271

On-chip integration of organic synthesis and HPLC/MS analysis for monitoring stereoselective transformations at the micro-scale
J. J. Heiland , R. Warias , C. Lotter , P. Fuchs , L. Mauritz , K. Zeitler , S. Ohla, D. Belder, Lab Chip 2017, 17, 76-81

Microchip HPLC separations monitored simultaneously by coherent anti-Stokes Raman scattering and fluorescence detection
D. Geissler, J. J. Heiland, C. Lotter, D. Belder, Microchim Acta 2017, 184, 315-321


Enantioselective reaction monitoring utilizing two-dimensional heart-cut liquid chromatography on an integrated microfluidic chip
C. Lotter, E. Poehler, J. J. Heiland, L. Mauritz, D. Belder, Lab Chip 2016, 16, 4648-4652

Evaluation of Pressure Stable Chip-to-Tube Fittings Enabling High-Speed Chip-HPLC with Mass Spectrometric Detection
C. Lotter, J. J. Heiland, V. Stein, M. Klimkait, M. Queisser, D. Belder, Anal. Chem. 2016, 88, 7481–7486

Integrated on-chip mass spectrometry reaction monitoring in microfluidic devices containing porous polymer monolithic columns
C. Dietze, S. Schulze, S. Ohla, K. Gilmore, P. Seeberger, D. Belder, Analyst 2016, 141, 5412-5416

Chip-based electrochromatography coupled to ESI-MS detection
C. Dietze, C. Hackl, R. Gerhardt, S. Seim, D. Belder, Electrophoresis 2016, 37, 1345-1352

HPLC-MS with Glass Chips Featuring Monolithically Integrated Electrospray Emitters of Different Geometries
C. Lotter, J. J. Heiland, S. Thurmann, L. Mauritz, D. Belder, Anal. Chem. 2016, 88, 2856–2863

Analysis of Enantioselective Biotransformations Using a Few Hundred Cells on an Integrated Microfluidic Chip
K. M. Krone, R. Warias, C. Ritter, A. Li, C. G. Acevedo-Rocha, M. T. Reetz, D. Belder, JACS 2016, 138, 2102–2105

A chip-integrated highly variable thermal flow rate sensor
C. Hoera, M. M. Skadell, S. A. Pfeiffer, M. Pahl, Z. Shu, E. Beckert, D. Belder, Sensors and Actuators B: Chemical 2016, 225, 42-49


Rapid prototyping of microfluidic chips for dead-volume-free MS coupling
C. Dietze, T. Scholl, S. Ohla, J. Appun, C. Schneider, D. Belder
Anal. Bioanal. Chem. 2015, 407, 8735-8743

Two-photon excitation in chip electrophoresis enabling label-free fluorescence detection in non-UV transparent full-body polymer chips
D. Geissler, D. Belder, Electrophoresis 2015, 36, 2976-2983

Fast electrically assisted regeneration of on chip SERS substrates
T. A. Meier, E. Poehler, F. Kemper, O. Pabst, H. G. Jahnke, E. Beckert, A. Robitzki, D. Belder, Lab Chip 2015, 15, 2923-2927

Chip-Based High-Performance Liquid Chromatography for High-Speed Enantioseparations
S. Thürmann, C. Lotter, J. J. Heiland, B. Chankvetadze, D. Belder, Anal. Chem. 2015, 87, 5568–5576

Chip-Based Free-Flow Electrophoresis with Integrated Nanospray Mass-Spectrometry
C. Benz, M. Boomhoff, J. Appun, C. Schneider, D. Belder, Angew. Chem. Int. Ed. 2015, 54, 2766-2770

On-chip monitoring of chemical syntheses in microdroplets via surface-enhanced Raman spectroscopy
T. A. Meier, R. J. Beulig, E. Klinge, M. Fuss, S. Ohla, D. Belder, Chem. Commun. 2015, 51, 8588-8591

An integrated microfluidic chip enabling control and spatially resolved monitoring of temperature in micro flow reactors
C. Höra, S. Ohla, Z. Shu, E. Beckert, S. Nagl, D. Belder, Anal. Bioanal. Chem. 2015, 407, 387-396

Surface modification of PDMS microfluidic devices by controlled sulfuric acid treatment and the application in chip electrophoresis
L. Gitlin, P. Schulze, S. Ohla, H.J. Bongard, D. Belder, Electrophoresis 2015, 36, 449-456


High-performance liquid chromatography on glass chips using precisely defined porous polymer monoliths as particle retaining elements
S. Thürmann, L. Mauritz, C. Heck, D. Belder, J. Chromatogr. A 2014,
1370, 33-39

Phase-optimized chip-based liquid chromatography
S. Thürmann, D. Belder, Anal. Bioanal. Chem. 2014, 406, 6599-6606

Rapid Prototyping of Electrochromatography Chips for Improved Two-Photon Excited Fluorescence Detection
C. Hackl , R. Beyreiss , D. Geissler , S. Jezierski, D. Belder, Anal. Chem. 2014, 86, 3773–3779

A low pressure on-chip injection strategy for high-performance chip-based chromatography
S. Thurmann, A. Dittmar, D. Belder, J. Chromatogr. A 2014, 1340, 59–67

Improving sensitivity in microchip electrophoresis coupled to ESI-MS/MS on the example of a cardiac drug mixture
F. Schwarzkopf, T. Scholl, S. Ohla, D. Belder, Electrophoresis 2014, 35, 1880-1886


Integrating continuous microflow reactions with subsequent micropreparative separations on a single microfluidic chip
S. Jezierski, V. Tehsmer,  S. Nagl,  D. Belder, Chem. Commun. 2013, 49, 11644-11646

Label-free fluorescence detection of aromatic compounds in chip electrophoresis applying two photon excitation and time-correlated single photon counting
R. Beyreiss, D. Geißler, S. Ohla, S. Nagl, T. N. Posch, D. Belder, Anal. Chem. 201385, 8150-8157

Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring 
L. Gitlin, C. Hoera, R. J. Meier, S. Nagl, D. Belder, Lab Chip 2013, 13, 4134-4141

Protein–protein interaction analysis in single microfluidic droplets using FRET and fluorescence lifetime detection
C. Benz, H. Retzbach, S. Nagl,  D. Belder, Lab Chip 2013, 13, 2808-2814

Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing 
S. Jezierski, A.S. Klein, C. Benz, M. Schaefer, S. Nagl,  D. Belder, Anal. Bioanal. Chem. 2013, 5381-5386

Microfluidic free-flow electrophoresis chips with an integrated fluorescent sensor layer for real time pH imaging in isoelectric focusing
S. Jezierski ,  D. Belder, S. Nagl, Chem. Commun. 2013, 49, 904-906

Crossing the Border towards Deep UV Time-Resolved Microscopy of Native Fluophores
M. Koenig, S. Tannert, T. Schoenau, K. Lauritsen, F. Koberling, R. Erdmann, R. Beyreiss, S. Nagel, D. Belder, Biophysical Journal 2013104, 667A-667A


Chip-based separation devices coupled to mass spectrometry 
S. Ohla, D, Belder, Current Opinion in Chemical Biology 2012, 16, 453–459

Monitoring on-Chip Pictet-Spengler Reactions by Integrated Analytical Separation and Label-Free Time-Resolved Fluorescence
S. Ohla,  R. Beyreiss, S. Fritzsche, P. Glaser, S. Nagl, K. Stockhausen, C. Schneider,  D. Belder*, Chem. Eur. J. 201218, 1240 – 1246

Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning
S. Köhler, S. Nagl, S. Fritzsche, D. Belder*, Lab Chip 2012, 12, 458-463

Micro free-flow electrophoresis with injection molded chips
S. Köhler, C. Benz, H. Becker, E. Beckert, V. Beushausen D. Belder*, RSC Adv. 2012, 2, 520-525

Poly(ethylene glycol)-coated microfluidic devices for chip electrophoresis
M. Schulze, D. Belder*, Electrophoresis 2012, 33, 370–378


Label-free analysis in chip electrophoresis applying deep UV fluorescence lifetime detection
R. Beyreiss, S. Ohla, S. Nagl, D. Belder*, Electrophoresis 2011, 32, 3108–3114

Multistep liquid-phase lithography for fast prototyping of microfluidic free-flow-electrophoresis chips
S. Jezierski, L. Gitlin, S. Nagl, D. Belder*, Anal. Bioanal. Chem. 2011, 401, 2651–2656

Asymmetric Organocatalysis and Analysis on a Single Microfluidic Nanospray Chip
S. Fritzsche, S. Ohla, P. Glaser, D.S. Giera, M. Sickert, C. Schneider, D. Belder*, Angew. Chem. Int. Ed. 2011, 50, 9467 –9470

Microfluidic chips for chirality exploration 
S. Nagl, P. Schulze, S. Ohla, R. Beyreiss, L. Gitlin, D. Belder*, Anal. Chem. 2011, 83, 3232–3238

Rapid quantitative determination of ephedra alkaloids in tablet formulations and human urine by microchip electrophoresis
D. Belder*, K. Tolba, S. Nagl, Electrophoresis 2011, 32, 440-447

Chip electrophoresis of active banana ingredients with label-free detection utilizing deep UV native fluorescence and mass spectrometry 
S. Ohla, P. Schulze, S. Fritzsche, D. Belder*, Anal. Bioanal. Chem. 2011, 399, 1853-1857

PDMS free-flow electrophoresis chips with integrated partitioning bars for bubble segregation 
S. Köhler, C. Weilbeer, S. Howitz, H. Becker, V. Beushausen, D. Belder*, Lab Chip 2011, 11, 309-314


An integrated on-chip sirtuin assay
S. Ohla, R. Beyreiss, G.K E. Scriba, Y. Fan, D. Belder*,  Electrophoresis 2010, 31, 3263-3267

Screening in One Sweep using the Slipchip
D. Belder, Angew. Chem. Int. Ed. 2010, 49, 6484–6486

A New Weakly Basic Amino-Reactive Fluorescent Label for Use in Isoelectric Focusing and Chip Electrophoresis
P. Schulze, M. Link, M. Schulze, S. Thuermann, O.S. Wolfbeis, D. Belder, Electrophoresis 201031, 2749–2753

Chip electrophoresis with mass spectrometric detection in record speed
S. Fritzsche, P. Hoffmann D. Belder*, Lab Chip 2010, 10, 1227–1230


Spray Performance of Microfluidic Glass Devices with Integrated Pulled Nanoelectrospray Emitters
P. Hoffmann, M. Eschner, S. Fritzsche, D. Belder*, Anal. Chem. 2009, 81, 7256-7261

Progress in microchip enantioseparations
S. Nagl, P. Schulze, M. Ludwig  D. Belder*, Electrophoresis 2009, 30, 2765-2772

Towards an Integrated Chemical Circuit
D. Belder, Angew. Chem. Int. Ed. 2009, 48, 3736–3737

New diode laser-excitable green fluorescent label and its application to detection of bovine serum albumin via microchipelectrophoresis, 
M. Link, P. Schulze, D. Belder*, O. Wolfbeis*, Microchim. Acta 2009, 166, 183-188

Label-free fluorescence detection for capillary electrophoresis and microfluidic chip-based techniques
P. Schulze, D. Belder*, Anal. Bioanal. Chem. 2009, 393, 515-525

Rapid replication of master structures by double casting with PDMS
L. Gitlin, P. Schulze, D. Belder*, Lab Chip 2009, 9, 3000-3002


Impact of laser excitation intensity on deep UV fluorescence detection in microchip electrophoresis
P. Schulze, M. Ludwig, D. Belder, Electrophoresis 2008, 29, 4894-4899


Fast quantitative determination of diuretic drugs in tablets and human urine by microchip electrophoresis with native fluorescence detection
K. Tolba, D. Belder, Electrophoresis 2007, 28, 2934-2941

Microfluidic glass chips with an integrated nanospray emitter for coupling to a mass spectrometer
P. Hoffmann, U. Haeusig, P. Schulze, D. Belder, Angew. Chem. Int. Ed. 2007, 46, 4913-4916

Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis
P. Schulze, M. Schuettpelz, M. Sauer, D. Belder, Lab Chip 2007, 7, 1841-1844


Coating of powder-blasted channels for high-performance microchip electrophoresis
D. Belder, F. Kohler, M. Ludwig, K. Tolba, N. Piehl, Electrophoresis 2006, 27, 3277-3283

Integrating chemical synthesis and analysis on a chip
D. Belder, Anal. Bioanal. Chem. 2006, 385, 416-418

Enantioselective catalysis and analysis on a chip
D. Belder, M. Ludwig, L.W. Wang, M.T. Reetz, Angew. Chem. Int. Ed. 2006, 45, 2463-2466


Deep UV laser-induced fluorescence detection of unlabeled drugs and proteins in microchip electrophoresis
P. Schulze, M. Ludwig, F. Kohler, D. Belder, Analytical Chemistry 2005, 77, 1325-1329

Microfluidics with droplets
D. Belder, Angew. Chem. Int. Ed. 2005, 44, 3521-3522


Subsecond chiral separations on a microchip
N. Piehl, M. Ludwig, D. Belder, Electrophoresis 2004, 25, 3848-3852

Separation of fluorescein isothiocyanate-labeled amines by microchip electrophoresis in uncoated and polyvinyl alcohol-coated glass chips using water and dimethyl sulfoxide as solvents of background electrolyte
S.J.O. Varjo, M. Ludwig, D. Belder, M.L. Riekkola, Electrophoresis 2004, 25, 1901-1906


Surface modification in microchip electrophoresis
D. Belder, M. Ludwig, Electrophoresis 2003, 24, 3595-3606

High-speed chiral separations on a microchip with UV detection
M. Ludwig, F. Kohler, D. Belder, Electrophoresis 2003, 24, 3233-3238

Microchip electrophoresis for chiral separations
D. Belder, M. Ludwig, Electrophoresis 2003, 24, 2422-2430

Coated microfluidic devices for improved chiral separations in microchip electrophoresis
M. Ludwig, D. Belder, Electrophoresis 2003, 24, 2481-2486


Poly(vinyl alcohol)-coated microfluidic devices for high-performance microchip electrophoresis
D. Belder,  A. Deege, F. Kohler, M. Ludwig, Electrophoresis 2002, 23, 3567-3573

Design and performance of a microchip electrophoresis instrument with sensitive variable-wavelength fluorescence detection
D. Belder,  A. Deege,  M. Maass, M. Ludwig, Electrophoresis 2002, 23, 2355-2361





letzte Änderung: 12.05.2021